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Jul 26, 2016Open Access
Targeted mutagenesis by
zinc-finger nucleases (ZFNs) can be used to generate knock-out mammalian cell
lines with high efficiency. A number of different methods have been developed
for the design and assembly of gene-specific ZFNs, making them easily accessible
to researchers. In this study, we used ZFNs assembled through the CoDA
(context-dependent assembly) platforms to generate mutant caprine fetal fibroblasts
cells for the BLG ...
Jun 17, 2016Open Access
We previously proposed the existence of DNA to DNA transcription in
eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA)
transcript is produced and released from the genome remains unknown. We once
speculated that the mechanism of DNA to DNA transcription might be similar to
that of DNA to RNA transcription, but now we propose that endonuclease dependent
transcript cutout may be a possible mechanism of DNA to D ...
Jun 13, 2016Open Access
The objective of this review was to critically present and evaluate recent
investigations into nanotechnology and it’s applications in reconstructive
surgery. In addition, this review aims to looks at a plethora of applications
with nanotechnology in the subject area of reconstructive surgery. The Medline
and PubMed databases were searched for clinical trial and case report
publications dealing with reconstructive
surgeries involv ...
May 17, 2016Open Access
Till now, in biological sciences, the term, transcription, mainly refers
to DNA to RNA transcription. But our recently published experimental findings
obtained from Plasmodium falciparum strongly
suggest the existence of DNA to DNA transcription in the genome of eukaryotic
cells, which could shed some light on the functions of certain noncoding DNA in
the human ...
Apr 18, 2016Open Access
Site-directed mutagenesis refers to a man-made molecular biology method that
is used to make genetic alterations in the DNA sequence of a gene of interest. But
based on our recently published experimental findings, we propose that “natural
site-directed mutagenesis” might exist in eukaryotic cells, which is triggered by
harmful agents and co-directed by special transcription hotspots and mutation-contained
intranuclear primers.
Mar 14, 2016Open Access
It is well
known that cyclins are a family of proteins that control cell-cycle progression
by activating cyclin-dependent kinase. Based on our experimental results, we
propose here a novel hypothesis that certain amplified genomic-DNA fragments
(AGFs) may also be required for the cell cycle progression of eukaryotic cells
and thus can be named as
cell-cycle-associated AGFs (CAGFs). Like fluctuation in cyclin levels during
cell cycle progression, these CAGFs are amplified and degraded at differen...
Feb 18, 2016Open Access
Bone marrow mesenchymal stem cells
(BMSCs) transplantation has emerged as a promising treatment for various central
nervous system diseases. However, oxidative stress results in poor survival of transplanted
BMSCs in injury central nervous system (CNS). Basic fibroblast growth factor (bFGF)
has been reported to have anti-apoptotic properties in serial of cells in oxidative
stress. This study was designed to investigate protective effect and potential mechanisms
of bFGF against hydrogen peroxide ...
Apr 24, 2015Open Access
The mechanism formation of colonies and biofilms of bacteria and yeasts are studied always of great interest. The aim of the presented work was transmission and scanning electron microscopic analysis contacts between cells of bacteria and yeast in biomofilms on natural structures and inorganic surface, as a result of formation of close contacts between a cellular wall, a fringe, cross-pieces, symplasts and cells of Escherichia coli, Shigella flexnerii Salmonella of typhi, Salmon...
Jun 05, 2014Open Access
In this study, the chromosome karyotype of Plumbago auriculata was analyzed by using a chro-mosome mounting technique with the primary root tip of seed germination. The aim was to find out the most suitable method for Plumbago auriculata by comparing the effect of different pretreatment and dissociation time on chromosome. The results showed that the best pretreatment was 0.05% colchicine solution under 0℃ - 4℃ or 2 h and then 1 mol/L HCL under 60℃ water for 6 min. Karyotype analysis of Plumbago...
May 29, 2014Open Access
For obtainment of the isolated sterile intestine, inbred lines of mice C3HA, CBA, C57BL/6, BalB/c are used at same time; it is carried out transplantation of small and large intestines of embryons corresponding lines of mice subcutaneously in adult singen mice. In capacite of test objects we make use of cultures of Salmonella typhimurium. The side of organ like kysts of isolated sterile of small and large intestines’ models on the histological and transmission electron-microscopic (TEM) preparat...
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